Rheumatoid arthritis
Many laboratories measure rheumatoid factor (RF) with nephelometry or turbidomet. However, these methods don't distinguish between the different RF isotypes, and RF IgA and IgM in particular can give important additional diagnostic guidance.1-6
The EliA™ Rheumatoid arthritis panel offers a full range of fully automated RF isotypes plus the best-in-class CCP2 - providing early diagnostic guidance and potentially improved outcomes for patients with rheumatoid arthritis.
The tests available to support the diagnosis of RA are:
EliA RF IgM (14-5600-01)
EliA RF IgM - best-in-class performance and fully automated10
Sensitivity and specificity of EliA RF IgM and 4 commercial ELISAs for RF IgM, in 100 RA patients and 178 disease controls. Sensitivity and specificity of RF tests are highly dependent on the patients panel.
Sensitivity and specificity of EliA RF IgM and 4 commercial ELISAs for RF IgM, in 100 RA patients and 178 disease controls. Sensitivity and specificity of RF tests are highly dependent on the patients panel.
Cut-off/measuring range
| Negative | Equivocal | Positive | Measuring range | |
|---|---|---|---|---|
| EliA RF IgM | <3.5 GPL-U/ml | 3.5-5 GPL-U/ml | >5 GPL-U/ml | 0.4-≥200 U/ml |
Coating
- Aggregated rabbit IgG antigen
Dilution
- 1:100
Sample material
- Serum, plasma (EDTA, citrate)
EliA RF IgA (14-5601-01)
EliA RF IgA - exceptional specificity10
Sensitivity and specificity of EliA RF IgA and 4 commercial ELISAs for RF IgA, in 100 RA patients and 178 disease controls. Sensitivity and specificity of RF tests are highly dependent on the patients panel.
Sensitivity and specificity of EliA RF IgA and 4 commercial ELISAs for RF IgA, in 100 RA patients and 178 disease controls. Sensitivity and specificity of RF tests are highly dependent on the patients panel.
Cut-off/measuring range
| Negative | Equivocal | Positive | Measuring range | |
|---|---|---|---|---|
| EliA RF IgA | <14 GPL-U/ml | 14-20 GPL-U/ml | >20 GPL-U/ml | 0.2-≥214 U/ml |
Coating
- Aggregated rabbit IgG antigen
Dilution
- 1:50
Sample material
- Serum, plasma (EDTA, citrate)
EliA RF IgG (14-5617-01)
EliA RF IgG - excellent performance10
Study conducted in 100 clinically defined Rheumatoid Arthritis samples; 177 sera from disease controls (84 Connective tissue disease; 8 Seronegative Spondylarthritis; 17 Psoriatic Arthritis; 20 Crohn's disease; 20 Ulcerative colitis; 28 Non-inflammatory rheumatic diseases).
Study conducted in 100 clinically defined Rheumatoid Arthritis samples; 177 sera from disease controls (84 Connective tissue disease; 8 Seronegative Spondylarthritis; 17 Psoriatic Arthritis; 20 Crohn's disease; 20 Ulcerative colitis; 28 Non-inflammatory rheumatic diseases).
Cut-off/measuring range
| Negative | Equivocal | Positive | Measuring range | |
|---|---|---|---|---|
| EliA RF IgA | <28 GPL-U/ml | 28-40 GPL-U/ml | >40 GPL-U/ml | 0.6-≥510 U/ml |
Coating
- Rabbit IgG antigen
Dilution
- 1:50
Sample material
- Serum, plasma (EDTA, citrate)
EliA CCP (14-5515-01)
EliA CCP - excellent performance10
Study conducted in 82 clinically defined Rheumatoid Arthritis samples; 450 sera from disease controls (120 Connective tissue disease; 185 Infection controls; 33 Osteoarthritis; 21 Vasculitides; 20 Autoimmune thyroid disease; 20 Crohn's disease; 20 Ulcerative colitis; 31 other diseases).
Study conducted in 82 clinically defined Rheumatoid Arthritis samples; 450 sera from disease controls (120 Connective tissue disease; 185 Infection controls; 33 Osteoarthritis; 21 Vasculitides; 20 Autoimmune thyroid disease; 20 Crohn's disease; 20 Ulcerative colitis; 31 other diseases).
Cut-off/measuring range
| Negative | Equivocal | Positive | Measuring range | |
|---|---|---|---|---|
| EliA CCP | <7 GPL-U/ml | 7-10 GPL-U/ml | >10 GPL-U/ml | 0.4-≥340 U/ml |
Coating
- Citullinated synthetic peptides (second generation antigen)
Dilution
- 1:100
Sample material
- Serum, plasma (EDTA, citrate)
Cut-off/measuring range, coating, dilution and sample material have been taken from respective Phadia 250 DFUs. The data is intended as a guide and should be treated accordingly. If you have any questions or uncertainty please contact your local service team or Laboratory Sales Representative.
References
- Heidari B. Caspian J Med 2011; 2: 161-170.
- Scott DL, et al. Lancet 2010; 376: 1094-1108.
- Bobbio-Pallavicini F, et al. Ann Rheum Dis 2007; 66(3): 302-307.
- De Angelis V, Meroni PL. Rheumatoid Factor. In: Autoantibodies, 2nd edition 2007, eds: Shoenfeld, Gershwin, Meroni. Elsevier, Amsterdam, pp 755-762.
- Jónsson T & Valdimarsson H. Ann Rheum Dis 1993; 52(2): 161-164.
- Shakiba Y, et al. Iran J Allergy Asthma Immunol 2014; 13(3): 147-156.
- Aletaha D, et al. Arthritis Rheum 2010; 62: 2569-81.
- Halldorsdottir HD, et al. Ann Rheum Dis 2000; 59: 149-51.
- Jaskowski TD, et al. J Rheumatol 2010; 37: 1582-8.
- Thermo Fisher Scientific. Internal Study.
