Connective Tissue Disease
Connective tissue diseases (CTDs) are a group of closely related multisystem conditions, with many overlapping clinical features.1 There are a number of different CTDs; each is potentially life-threatening. CTDs include systemic lupus erythematous (SLE), systemic sclerosis, Sjögren's syndrome, inflammatory muscle diseases and overlap-syndromes.2 The tests available to support the diagnosis of CTD are:
EliA™ CTD Screen
(14-5596-01)
The EliA CTD screen comprises 14 of the most clinically relevant markers associated with CTD. It even tests for antibodies that are difficult to detect with IFA, such as those to Ro, dsDNA, Rib-P and Jo-1. With the exception of Sm proteins and Native purified dsDNA, all markers are human recombinant antigen and therefore are associated with less false positives. EliA CTD screen is a diagnostically relevant screen with outstanding specificity and sensitivity3
| HEp-2 | EliA™ CTD Screen | ||
|---|---|---|---|
| Cut-off | 1:80 | 1:160 | Ration 1:0 |
| Sensitivity | 85.0% | 72.5% | 70.0% |
| Specificity | 54.9% | 69.2% | 90.1% |
| Positive predictive value (PPV) | 45.3% | 50.9% | 75.7% |
| Negative predictive value (NPV) | 89.3% | 85.1% | 87.2% |
Cut-off/measuring range
| Negative | Equivocal | Positive | Measuring range | |
|---|---|---|---|---|
| EliA CTD Screen | <0.7 ratio/td> | 0.7-1.0 ratio | >1.0 ratio | 0.03-≥32 ratio |
Coating
- Human recombinant U1RNP (RNP70, A, C), SS-A/Ro (60 kDa, 52 kDa), SS-B/La, Centromere B, Scl-70, Jo-1, Fibrillarin, RNA Pol III, Rib-P,PM-Scl, PCNA, Mi-2 proteins, Sm proteins and native purified DNA
Dilution
- 1:10
Sample material
- Serum, plasma (EDTA, citrate, heparin)
EliA Symphony
(14-5508-01)
EliA Symphony is a clinically relevant screen (PPV of 77% and NPV of 84%)[GON05]
| EliA™ Symphony Pos | EliA™ Symphony Neg | |
|---|---|---|
| SLE | 21 | 13 (including 7 patients with inactive disease) |
| Other CTD | 13 | 7 |
| Other AI Disease | 2 | 24 |
| Non AI Disease | 8 | 82 |
| Totals | 44 | 126 |
Cut-off/measuring range
| Negative | Equivocal | Positive | Measuring range | |
|---|---|---|---|---|
| EliA Symphony | <0.7 ratio | 0.7-1.0 ratio | >1.0 ratio | 0.03-≥32 ratio |
Coating
- Human recombinant U1RNP (70kDa, A, C), Ro (60kDa, 52kDa), La, Centromere B, Scl-70 and Jo-1 proteins, native purified Sm proteins
Dilution
- 1:100
Sample material
- Serum, plasma (EDTA, citrate, heparin)
EliA ANA
EliA dsDNA (14-5500-01)
EliA ssDNA (14-5629-01)
EliA U1RNP (14-5501-01)
EliA RNP70 (14-5511-01)
EliA SmDP (14-5624-01)
EliA Ro (14-5503-01)
EliA Ro52 (14-5598-01)
EliA Ro60 (14-5525-01)
EliA La (14-5504-01)
EliA CENP (14-5505-01)
EliA Scl-70S (14-5637-01)
EliA RNA Pol III (14-5599-01)
EliA Jo-1 (14-5507-01)
EliA Fibrillarin (14-5605-01)
EliA Rib-P (14-5521-01)
EliA PM-Scl (14-5602-01)
EliA PCNA (14-5603-01)
EliA Mi-2 (14-5604-01)
Cut-off/measuring range, coating, dilution and sample material have been taken from respective Phadia 250 DFUs. The data is intended as a guide and should be treated accordingly. If you have any questions or uncertainty please contact your local service team or Laboratory Sales Representative.
- Adebajo AO, et al. Collected reports on the rheumatic diseases. 2005. Available from www.arthritisresearchuk.org/~/media/Files/Education/Hands-On/IP03-Sep-2000.ashx; last accessed March 2015.
- Gaubitz M. Rheumatology (Oxford) 2006;45 (Suppl 3): iii3-4.
- Thermo Fisher Scientific. Internal Study.
- González C, et al. Clin Chim Acta 2005; 359:109-114.
