These questions have been provided as a reference to support your training. If you are unsure of how to proceed or would like further information please contact the support helpline or your local Laboratory Sales Representative.
Key tools including DFUs, certificates of analysis and safety data sheets can be accessed through the resources section of this website. Another key resource is the IDM manual which can be accessed through IDM.
ImmunoCAP?
40 µl is required for each test. However, the total sample volume needed is dependent on the type of sample tube used and dead volume for that specific tube.
EliA?
If the Phadia 250 instrument dilution is used the sampling amount depends on the dilution factor:
- 1:10 (one-step dilution) = 9 µ l of neat sample
- 1:50 or 1:100 (two-step dilution) = 20 µ l of neat sample
Note: The total sample volume needed depends on the type of sample tube used and the dead volume as for ImmunoCAP